|
KMID : 0380620090410040405
|
|
Korean Journal of Food Science and Technology
2009 Volume.41 No. 4 p.405 ~ p.412
|
|
|
Inhibitory Effect of Glycoprotein Isolated from Cudrania tricuspidata Bureau on Histamine Release and COX-2 Activity in RBL-2H3 Cells
|
|
Oh Phil-Sun
Lee Hye-Jin
Lim Kye-Taek
|
|
|
Abstract
|
|
|
|
The purpose of this study was to determine the inhibitory effect of a glycoprotein isolated from Cudraniatricuspidata Bureau (CTB glycoprotein, 75kDa) on immunoglobulin E (IgE)-induced allergic inflammation in RBL-2H3cells. This experiment evaluated the production of intracellular reactive oxygen species (ROS), the activities of mitogen-activated protein kinase (MAPK), transcription factor (c-jun), and cyclooxygenase (COX)-2, and histamine release in cells.The results showed that the CTB glycoprotein inhibited histamine release and COX-2 expression induced by IgE in thecells. The CTB glycoprotein also had suppressive effects on the expressions of ERK1/2, p38 MAPK, c-jun, and theproduction of intracellular ROS in IgE-treated RBL-2H3 cells. The activities of c-jun and COX-2 were collectively blockedby ERK1/2 inhibitor (PD98059) and p38 MAPK inhibitor (SKF86002), respectively. Hence, we speculate that CTBglycoprotein might be a component with potential use in the preparation of health supplements for the prevention ofallergic diseases.
|
|
|
KEYWORD
|
|
|
Cudrania tricuspidata Bureau glycoprotein, RBL-2H3 cells, histamine, cyclooxygenase-2
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|